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TECHNICAL APPROACHES TO THE STUDY OF EXTREMOPHILES

General Information

It is understood as an extreme environment in which the temperature, acidity, salinity, pressure or level of radiation are hostile to life, from an anthropocentric point of view. The organisms that live in these environments are called extremophiles and are so perfectly adapted to the environment that all its components work optimally in these extreme conditions. Among the extremophiles are thermophilic, halophilic, acidophilus and alkalophilic microorganisms, among others. The interest of his study has a basic aspect, related to the processes of evolution and origin of life on Earth and its possible presence in other planets, and another applied, given the large number of substances with biotechnological interest produced by these microorganisms. This course will present the most current techniques for studying extremophile microorganisms, from the biochemical characterization of their enzymes to the analysis of microbial communities inhabiting extreme environments.

  • Director: Josefa Antón Botella
  • Department: Physiology, Genetics and Microbiology. University of Alicante
  • Co-organized with Autonomous University of Madrid
  • Credits: 32 hours / 3.2 credits / ECTS
  • Date: from June 26 to June 29, 2017
  • Price: UA students: 150 Euros. Non UA students: 200 Euros
  • Venue: University of Alicante Campus.
  • Admission requirements: Intermediate English Level. Students of Physiology, Genetics, Microbiology, Agrochemistry and Biochemistry
  • Requirements for obtaining the diploma or certificate: Your grade in this class will depend on your class attendance, performance on a final exam and a presentation (team work)
  • All the classes are taught in English
  • Program

MONDAY 26

9:00 - 10:00 h   Course presentation
Josefa Antón Botella. Associate professor of Microbiology. University of Alicante

10:00 - 11.00 h  Opening lecture: CRISPR: from brine to brain.
Francisco J.M. Mojica. Associate professor of Microbiology. University of Alicante

11:00 - 11:30 h  Coffee break

11:30 - 14:30 h  Lab session: FISH (I)
José Luis Sanz Martín. Microbiology Professor. Autonomous University of Madrid
María Gomariz. Researcher. University of Alicante.
LA*: Sara Díaz.
                         

16:00 – 19:00 h  Lab session. Viruses from hypersaline environments (I). FISH (II).
Elina Roine. Programme in Molecular Virology. University of Helsinky
José Luis Sanz Martín. Microbiology Professor. Autonomous University of Madrid

 

 TUESDAY 27

08:30 - 10:45 h   Lab session. Gene transfer in thermophiles and halophiles (I)
José Berenguer Carlos. Microbiology Professor. Autonomous University of Madrid
María José Bonete Pérez. Biochemistry Professor.
University of Alicante
Julia Esclapez Espliego. Biochemistry Professor. University of Alicante
LA*: Ana López y Mercedes Sánchez.. Researchers. Autonomous University of Madrid

10:45 - 11:10 h  Coffee break

11:10 - 14:00 h  Lab session**. Electron microscopy of samples from extreme environments
Mª Asunción de los Ríos Murillo. CSIC Scientist . Institut of Natural Resources. CSIC
Jacek Wierzchos. CSIC Scientist . Institut of Natural Resources. CSIC

11:15 - 14:00 h   Computer room session**. Genomics and metagenomics of extreme environments
Manuel Martínez García. Associate professor of Microbiology. University of Alicante
Ana Belén Martín Cuadrado. Researcher. University of Alicante              

16:00 - 18:00 h Lab session. Extremophile culture (I)
Juan Miguel González Grau. CSIC Scientist . Instituto de Recursos Naturales y Agrobiología

18:00 - 19:00 h Lab session. Viruses from hypersaline environments (II)
Josefa Antón Botella. Associate professor of Microbiology. University of Alicante


WEDNESDAY 28

09:00 - 10:30 h  Invited talk: Metaproteomics of natural environments
Joseph Christie-Oleza. Natural Environment Research Council Fellow. University of Warwick

10:30 - 11:00 h Lab session. Extremophile culture (II)
Juan Miguel González Grau.  CSIC Scientist . Instituto de Recursos Naturales y Agrobiología

11:00 - 11:15 h Coffee break

11:15 - 14:00 h Lab session. Electron microscopy of samples from extreme environments**
Mª Asunción de los Ríos Murillo. CSIC Scientist . Institut of Natural Resources. CSIC
Jacek Wierzchos. CSIC Scientist . Institut of Natural Resources. CSIC

11:15 - 14:00 h Computer room session. Genomics and metagenomics of extreme environments**
Manuel Martínez García. Associate Professor of Microbiology. University of Alicante
Ana Belén Martín Cuadrado. Researcher. University of Alicante

15:30 - 16:30 h Viruses from hypersaline environments (III)
Elina Roine. Programa de Virología Molecular. Universidad de Helsinky

16:30 - 17:30 h Lab session. Recombinant expression and characterization of extremozymes
José Berenguer Carlos. Microbiology Professor. Autonomous University of Madrid
María José Bonete Pérez. Biochemistry Professor. University of Alicante
Julia Esclapez Espliego. Biochemistry Professor. University of Alicante
Rosa María Martínez-Espinosa. Associate Professor of Biochemistry. University of Alicante

17:30 - 19:00 h Applications of Extremophiles: Quorum sensing and quorum quenching enzymes
Inmaculada Llamas. Associate Professor of Microbiology. University of Granada
Marta Torres. Researcher.University of Granada
Inmaculada Sampedro. Ramón y Cajal Researcher. University of Granada
LA*: José Carlos Reina

 

THURSDAY 29

09:00 -10:00 h  Lecture: Microfluidics for (micro)biology
Aurelio Hidalgo. Department of Molecular Biology. Molecular Biology Center "Severo Ochoa"

10:00 - 11:00 h Lab session. Gene transfer in thermophiles and halophiles (II). Encapsulation of extremophiles in
                         microdroplets (I)

José Berenguer Carlos. Microbiology Professor. Universidad Autónoma de Madrid
Aurelio Hidalgo. Department of Molecular Biology. Molecular Biology Center "Severo Ochoa"

11:00 - 11:30 h Coffee break

11:30 -14:00 h  Lab session. Gene transfer in thermophiles and halophiles (III). Encapsulation of extremophiles in
                         microdroplets (II)

José Berenguer Carlos. Catedrático de Microbiología. Autonomous University of Madrid
Aurelio Hidalgo. Department of Molecular Biology. Molecular Biology Center "Severo Ochoa"

16:00 - 17:00 h General discussion

17:00 - 18:30 h Closing lecture. Microbiology of an oligotrophic extreme environment: the subsurface of the Iberian Pyrite Belt
Ricardo Amils. Microbiology Professor. Autonomous University of Madrid

 Laboratory supervisors (University of Alicante): Borja Aldeguer, Leticia Asensio, Mónica Lluesma, Loles Ramos y Cristina López
*LA: Laboratory assistants
** Simultaneous activities, in Small groups
All the activities will take place in the Laboratory Edificio Ciencias IV (SIGUA: 0004PB0018), except the Microscopy sessions (in the Servicios Técnicos de Investigación), the Metagenomic session (CI/INF2, SIGUA: 0010PB020), and the course presentation, general discussion and lectures (Sala de Juntas de la Facultad de Ciencias, SIGUA: 0007P1005). Before the microscopy sessions students will attend a short lecture in classroom 5 (SIGUA: 007PB076)

 

Enrollment

  • Pre-registration and registration information

If the number of students enrolled in the course does not exceed the minimum University of Alicante can resolve the non-celebration of the course.

 

Organized with:

 

 

 

 

Sponsors:

                                                 

 

                           

University of Alicante 'Rafael Altamira' Summer Courses


Cursos de Verano de la Universidad de Alicante "Rafael Altamira"
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